Application of deep eutectic solvent in ultrasound-assisted emulsification microextraction of quercetin from some fruits and vegetables

Kanberoğlu G. S., YILMAZ E., SOYLAK M.

JOURNAL OF MOLECULAR LIQUIDS, cilt.279, ss.571-577, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 279
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.molliq.2019.01.130
  • Dergi Adı: JOURNAL OF MOLECULAR LIQUIDS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.571-577
  • Anahtar Kelimeler: Deep eutectic solvent, Quercetin, Microextraction, UV-VIS spectrophotometry, LIQUID-LIQUID MICROEXTRACTION, SOLID-PHASE EXTRACTION, WATER SAMPLES, IONIC LIQUIDS, TRACE AMOUNTS, HPLC, LEAD, PRECONCENTRATION, TOXICITY, CATECHIN
  • Erciyes Üniversitesi Adresli: Evet

Özet

A deep eutectic solvent ultrasound-assisted emulsification microextraction (USAEME-DES) procedure for the separation and preconcentration of quercetin in vegetable and fruit samples has been established. An alcohol-based extraction has been applied as sample pre-treatment step prior to microextraction procedure. Quercetin was extracted into DES phase (tetrabutylammonium chloride: decanoic acid (TBACI-DA, 1:3 M ratio) and analyzed by UV-VIS spectrophotometry at 370 nm. For the quantitative recoveries of quercetin, optimal pH, sample solution volume, volume and kind of DES, and ultrasonication time were 3.0, 15 mL, 250 mu L. of 1:3 M ratio of TBACI-DA acid and 5 min, respectively. The influences of matrix constituents were also studied. The developed method has a limit of detection (LOD) of 18.8 mu g L-1 and a relative standard deviation (RSD) of 5.1%. The developed procedure was applied to analysis of vegetable and fruit samples, which prepared by alcohol based extraction step. (C) 2019 Elsevier B.V. All rights reserved.