Effects of Ozone and L-Carnitine on Kidney MDA, GSH, and GSHPx Levels in Acetaminophen Toxicity

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EROĞLU H. A., Makav M., ADALI Y., ÇİTİL M.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.26, sa.1, ss.127-134, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.9775/kvfd.2019.22456
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.127-134
  • Anahtar Kelimeler: Acetaminophen, Ozone, L-Carnitine, Nephrotoxicity, ACETYL-L-CARNITINE, INDUCED NEPHROTOXICITY, LIPID-PEROXIDATION, OXIDATIVE STRESS, THERAPY, ANTIOXIDANT, DAMAGE, LIVER, BLOOD, HEPATOTOXICITY
  • Erciyes Üniversitesi Adresli: Evet

Özet

This study aimed to determine the therapeutic effects of medical ozone and L-carnitine therapy on acetaminophen (APAP)-induced kidney damage by evaluating malondialdehyde (MDA), glutathione (GSH), and GSHPx levels. In this study, 56 rats were randomized into 8 groups with 7 rats in each group. Kidney injury was induced by the administration of a single dose N-acetyl-p-aminophenol (1 g/kg) orally.Therapeutic ozone (0.7 mg/kg) and L-carnitine (500 mg/kg) were administered intraperitoneally. After the therapy, the rat kidneys were homogenized, and the tissue MDA, GSH, and GSHPx levels were measured. Compared to the control groups, there were higher MDA levels in the kidney tissues only in the "APAP," "APAP + Ozone," and "APAP + Ozone + L-carnitine" groups (P<0.001). Besides, the decrease in the GSH and GSHPx levels of the kidney tissues in the study groups were significant compared to the control groups, and the highest decreases were observed in the "APAP" "APAP + Ozone" and "APAP + Ozone + L-carnitine" groups (P<0.001). Findings obtained from this study revealed that acetaminophen toxicity caused oxidative damage in the examined kidney tissues, and L-carnitine and/ or ozone applications for protective purposes decreased MDA levels, a product of lipid peroxidation, and increased tissue GSH levels thru GSHPx antioxidant enzyme activity. In this context, the most important protective effect was observed in the group where L-carnitine and ozone were administrated together.