Determination of 3,5-dimethylpyrazolium glyceroborate nitrification inhibitor in nitrogen fertilizer samples: HPLC-DAD method development and validation for 3,5-dimethylpyrazole


ŞAHAN S. , ŞAHİN U. , BAŞARAN M. , UZUN O. , GÜNEŞ A.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, cilt.1068, ss.277-281, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 1068
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.jchromb.2017.10.050
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Sayfa Sayısı: ss.277-281

Özet

3,5 - Dimethylpyrazolium glyceroborate is a nitrification inhibitor (a member of pyrazole derivatives) used for the fixation of nitrogen into the soil. In this study, an HPLC-DAD method was developed and validated for determination of 3,5 dimethylpyrazole in order to determine 3,5 - dimethylpyrazolium glyceroborate in fertilizer samples. For method development, analytical parameters like type of eluent solution and column filling material and device parameters like eluent flow rate, column oven temperature and measurement wavelength were all optimized. For method validation, implementations were performed for linearity, limit of detection (LOD), limit of quantification (LOQ), specificity, stability, intra- and inter -day precision and accuracy. The developed and validated method was used for inhibitor detection in nitrogenous fertilizers. Sample analyses were performed with 95.6-103.3% recovery rates and 0-4.61% relative errors.