Detection of Bovine Leukocyte Adhesion Deficiency (BLAD) Allele in Holstein Cows Reared in Kayseri Vicinity

AKYÜZ B., Ertugrul O., Agaoglu O. K.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.16, no.3, pp.519-521, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 3
  • Publication Date: 2010
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.519-521
  • Erciyes University Affiliated: Yes

Abstract

The purpose of this work was to study whether the bovine leukocyte adhesion deficiency (BLAD) allele was present in the Holstein cows reared in Kayseri vicinity. Blood samples were obtained from 136 Holstein cows. In order to determine the area of mutation in PCR products, the PCR products were digested with TaqI endonuclease enzyme. It was found that three of the 136 Holstein cows were BLAD carriers. The mutant BLAD allele frequency in the 136 Holstein cows was calculated as 2.2%..