ARCHIVES OF VIROLOGY, vol.167, no.9, pp.1831-1840, 2022 (SCI-Expanded)
Viral enteritis is a signifcant threat to domestic dogs. The two primary pathogens that cause viral enteritis in dogs are canine
coronavirus (CCoV) and canine parvovirus (CPV). In this study, we investigated the occurrence of CPV-2, CCoV, and canine
circovirus coinfection by characterizing circulating subtypes of CPV-2 in faecal samples from symptomatic dogs admitted
to veterinary clinics located in Ankara, Elazığ, Kayseri, and Kocaeli provinces of Turkey, between 2019 and 2022. Virus
detection by PCR and RT-PCR revealed that CPV-2 was present in 48 (77.4%) samples, and no other agents were detected.
Based on the occurrence of the codon GAT at positions 1276 to 1278 (coding for aspartate at residue 426) of VP2, all CPV-2
isolates were confrmed to be of the CPV-2b subtype. The complete genome sequences of two CPV-2b isolates showed a
high degree of similarity to and phylogenetic clustering with Australian and East Asian strains/isolates. The predominant
CPV strain circulating in the three diferent regions of Turkey was found to be a CPV-2b strain containing the amino acid
substitutions at Y324I and T440A, which commonly contribute to immune escape. This is the frst report of complete
genomic analysis of CPV-2 isolates circulating in symptomatic domestic dogs in Turkey. The evolution of CPV-2 has raised
questions about the efcacy of current vaccination regimes and highlights the importance of monitoring the emergence and
spread of new CPV-2 variants.