Aptamer-Conjugated Multifunctional Nanoflowers as a Platform for Targeting, Capture, and Detection in Laser Desorption Ionization Mass Spectrometry


Ocsoy I., GULBAKAN B., SHUKOOR M. I. , XIONG X., Chen T., POWELL D. H. , ...More

ACS NANO, vol.7, no.1, pp.417-427, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.1021/nn304458m
  • Journal Name: ACS NANO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.417-427
  • Keywords: hybrid nanoparticles, nanoflower, aptamer, ATP, cancer cell, mass spectrometry, IRON-OXIDE NANOPARTICLES, GOLD NANOPARTICLES, GRAPHENE OXIDE, MAGNETIC NANOPARTICLES, SELECTIVE ENRICHMENT, SMALL MOLECULES, ION FORMATION, BINDING-SITE, NANO-FLARES, LIVE CELLS
  • Erciyes University Affiliated: No

Abstract

Although many different nanomaterials have been tested as substrates for laser desorption and ionization mass spectrometry (LOWS), this emerging field still requires more efficient multifuncional nanomaterials for targeting, enrichment, and detection. Here, we report the use of gold manganese oxide (Au@MnO) hybrid nanoflowers as an efficient matrix for LDI-MS. The nanoflowers were also functionalized with two different aptamers to target cancer cells and capture adenosine triphosphate (ATP). These nanoflowers were successfully used for metabolite extraction from cancer cell lysates. Thus, in one system, OUT multifunctional nanoflowers can (1) act as an ionization substrate for mass spectrometry, (2) target cancer cells, and (3) detect and analyze metabolites from cancer cells.