This study was designed to investigate whether kits to measure circulating cardiac troponin-I (cTn-I) and cardiac troponin-T (cTn-T) can be used to determine myocardial cell damage in cattle with traumatic reticuloperitonitis (TRP). Twenty cattle with TRP were compared with 10 clinically healthy cattle. cTn-I and cTn-T were determined qualitatively and cTn-I was determined quantitatively; biochemical analyses were also performed on both groups. The mean serum concentrations of total protein, globulin, glucose and calcium, and the mean activities of creatine kinase MB, aspartate aminotransferase, lactate dehydrogenase and gamma-glutamyl transferase were higher in the cattle with TRP than in the control group. The cTn-I and cTn-T kits both gave positive results in three of the cattle with TRP and the quantitative measurement of cTn-I was positive in 11 of the TRP cases. Both tests were negative in the healthy cattle.