The Suppressive Effect of Novel Hydrazones-Tethered Imidazoles in HCT-116 and HT-29 Colorectal Cancer Cells: Synthesis, Biological Activity and Molecular Modeling Studies


Kekecmuhammed H., Tapera M., Sahin K., Sever B. O., Anber A. M., Bora R. E., ...Daha Fazla

CHEMISTRYSELECT, cilt.9, sa.19, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 19
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1002/slct.202303108
  • Dergi Adı: CHEMISTRYSELECT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Erciyes Üniversitesi Adresli: Evet

Özet

A series of hybrid compounds containing both the imidazole ring and the hydrazone moiety have been synthesized. Synthesized compounds were characterized by various spectral techniques, including FT-IR, 1H-NMR, 13C-NMR, and HRMS. The compounds were evaluated for their antiproliferative activities on colorectal cancer cells HCT-116 and HT-29 in a time-dependent manner. Among them, some compounds exhibited remarkable anti-cancer activity with a less cytotoxic effect on non-cancerous cell lines, especially HRK-2 with IC50 value of 1.35 +/- 0.18 mu M in HCT-116 cells and HRK-5 with IC50 value of 2.67 +/- 0.61 mu M in HT-29 cells. Investigations of colon cancer cell death were performed, and the most active compounds were found to trigger cell death via nuclear localization and induce S phase arrest of the colon cancer cell. Moreover, molecular modeling studies for the synthesized compounds was performed to predict their binding affinities toward the active site of BCL-2. The findings of the molecular modeling investigations were highly consistent with those of the cytotoxicity results. A set of hydrazone-tethered imidazoles was synthesized by a three-component Bignelli-like reaction. Synthesized compounds were evaluated as anti-colon cancer agents, and their binding mechanisms were investigated. image