Research Information System
Small Ruminant Research, vol.248, 2025 (SCI-Expanded)
If antimicrobial treatment of infections by multidrug-resistant bacteria fails, the use of bacteriophages comes into focus. This study was a proof of concept (POC) and investigated if carefully selected E.coli bacteriophages (Φ IA-38) are superior to routine antibiotic post-infection treatment of E. coli-induced secretory diarrhea in neonatal lambs. Furthermore, the prophylactic application of Φ IA-38 was also investigated in the same trial. For this aim, a Φ IA-38 was isolated from sewage samples from a small sheep farm. The isolate had broad lytic activity against various E. coli strains, and its genome contains no antibiotic resistance or virulent genes. For the clinical part, 36 Awassi lambs (20 males, 16 females), aged 1–7 days and weighing 3.2–5.7 kg, were randomly allocated to four experimental groups comprising three treatment groups and one control group. Briefly, group 1 was the control group that was not infected nor received bacteriophages; group 2 was infected and treated 24 h later with a combination of procaine penicillin and dihydrostreptomycin; group 3 was treated prophylactically 12 h before infection, and group 4 was infected and treated 1 day after infection. A single dose of E. coli K99 (0.1 mL; 10⁷ CFU/mL) was given orally for infection. Lambs intramuscularly received 0.1 mL of phage Φ IA-38 (10⁷ PFU/mL; MOI:1) for 3 days for phage therapy. Next to routine hematology and clinical biochemistry, blood concentrations of Serum amyloid A (SAA), Haptoglobin (Hpt), immunoglobulin M (IgM), and immunoglobulin G (IgG) were determined to estimate the magnitude of inflammatory and general immune responses. None of the lambs died. All other lambs had mild to severe diarrhea except for the control group. SAA concentrations in the animals of Group 2 were increased at all time points after day 1, whereas the Hpt levels had decreased on day 10 in Group 4 (P < 0.05). IgM levels on day 7 were higher in phage-treated Groups 3 and 4, compared to the negative control and the pen-strep-treated other groups (P < 0.05). The IgG levels were significantly increased only in the animals of Group 3 on the 7th and 10th days (P <0.05). SAA concentrations in the animals treated with antibiotics (Group 2) were increased at all time points after day 1. In the Φ IA-therapeutically treated animals (Group 4), Hpt levels were already lowest and had significantly (p < 0.05) decreased by day 10. IgM levels on day 7 were higher in the prophylactic (Group 3) and therapeutic Φ IA-38- treated groups (Group 4) compared to the negative control and the pen-strep-treated groups (P < 0.05). The IgG levels were significantly increased only in the animals that had received the bacteriophages prophylactically before infection on the 7th and 10th days (P < 0.05). Prophylactic and therapeutic IA-38 treatment reduced significantly (p < 0.05) E. coli K99 counts in the feces of infected lambs, similar to the antibiotic treatment. However, complete E. coli K 99 elimination was not reached by day 10. In conclusion, we showed the POC for Φ IA-38 therapy, but dosing and treatment duration were insufficient to eliminate all E. coli K99. Further studies are warranted to optimize the elimination of multi-resistant Enterotoxigenic E. coli (ETEC) infection of the intestinal tract of neonatal lambs.