Trace metal content of nine fish species harvested from the Black and Aegean Seas were determined by microwave digestion and atomic absorption spectroscopy (MD-AAS). Verification of the MD-AAS method was demonstrated by analysis of standard reference material (NRCC-DORM-2 dogfish muscle). Trace metal content in fish samples were 0.73-1.83 mu g/g for copper, 0.45-0.90 mu g/g for cadmium, 0.33-0.93 mu g/g for lead, 35.4-106 mu g/g for zinc, 1.28-7.40 mu g/g for manganese, 68.6-163 mu g/g for iron, 0.95-1.98 mu g/g for chromium, and 1.92-5.68 mu g/g for nickel. The levels of lead and cadmium in fish samples were higher than the recommended legal limits for human consumption. (C) 2007 Elsevier Ltd. All rights reserved.