Akademik Veri Yönetim Sistemi
Cryobiology, cilt.68, sa.3, ss.349-353, 2014 (SCI-Expanded)
This study was designed to evaluate the in vitro effects of l-carnitine and glutamine (Gln) on the sperm quality parameters of liquid-stored rabbit semen maintained up to 24. h at 5. °C. Pooled and extended ejaculates were divided into two equal portions. l-Carnitine doses of 0.5, 1 and 2. mM were added to the first portion, and glutamine was added at the same doses to the second portion. All samples were cooled to 5. °C and examined at 0, 6, 12 and 24. h of liquid storage. Supplementation of the semen extender with three different doses of l-carnitine provided significant increases in the percentage of motile sperm at 12. h (P<. 0.01), and 24. h (P<. 0.001) and enabled significant protection of the sperm plasma membrane (P<. 0.01) at 12 and 24. h of cool-storage, in comparison to the control samples. Only the 2. mM dose of l-carnitine significantly (P<. 0.01) decreased the rate of acrosomal damage when compared to the control group. Furthermore, all doses of Gln caused a significant (P<. 0.01) decrease in acrosomal damage at 6. h, and provided significant improvement (P<. 0.01) in sperm motility, acrosomal and plasma membrane integrities at 12 and 24. h of liquid storage, when compared to the controls. In conclusion, the supplementation of liquid-stored rabbit semen with l-carnitine and Gln provided a protection for sperm against cool storage-induced functional and structural damages. © 2014 Elsevier Inc.