Specific phobia which is a state of excessive and constant fear although individuals know it is illogical. During phobia in individuals there is several changes. This study based on recorded pupil video during phobia and analysis of changes in pupil size using image processing and signal processing algorithms on this video. Feature extraction are made from the signals obtained from the pupil dimensions. These features were statistically analyzed to determine differences between groups. As a result of this analysis laid out attributes will be important in the diagnosis and monitoring of phobia.