Akademik Veri Yönetim Sistemi
Erciyes Üniversitesi Veteriner Fakültesi Dergisi, cilt.22, sa.2, ss.103-111, 2025 (TRDizin)
Mastitis is a disease that causes significant economic losses in dairy farms and is caused by many microorganisms and physical trauma. In this study, it was aimed to detect subclinical mastitis (SCM) using the California Mastitis Test (CMT) and bacteriological analysis in dairy cattle and to determine the antibiotic susceptibilities of the obtained isolates. For this purpose, a total of 400 milk samples from 100 cattle were screened with CMT. The CMT-positive samples were subjected to bacteriological analysis. The samples were inoculated onto 7% sheep blood agar and incubated at 37°C for 24-96 h in aerobic/microaerobic/anaerobic atmospheres. Colonies grown were evaluated. The identification of the obtained isolates was performed by phenotypic tests, MALDI-TOF MS, and 16S rRNA gene sequencing. The susceptibilities of the isolates to nine antibiotics were determined by the disk diffusion method. The prevalence of SCM was detected as 25.75% (103/400) for the CMT results. In the bacteriological analysis, 74 (71.8%) of 103 milk samples from 60 CMT-positive animals were detected as positive, and 75 isolates were obtained. The most frequently defined bacteria were coagulase-negative Staphylococcus (n=25), Staphylococcus aureus (n=17), and Trueperella pyogenes (n=8). The highest antibiotic resistance among all isolates was detected to gentamicin (48%) and tetracycline (32%), while Staphylococcus spp. isolates were determined to be highest penicillin (45.2%) and gentamicin (38.1%). In conclusion, the current study revealed that there may be a need for dairy farms in this region to develop useful strategies for the treatment/control of SCM, considering the pathogenic bacteria and high antibiotic resistance.