Akademik Veri Yönetim Sistemi
Indian Journal of Pediatrics, 2025 (SCI-Expanded, Scopus)
Objectives: To investigate the clinical, culture-based, histopathological and molecular aspects, genotyping and antibiotic susceptibility patterns of Helicobacter pylori (H. pylori) infection in dyspeptic pediatric patients. Methods: Biopsy samples from 245 dyspeptic pediatric patients were used, with four samples taken from each patient. Two samples were used for bacteriological/molecular analysis, while the others were used for histopathological examination. The presence of the virulence genes babA2, cagA, cagE, iceA and vacA in H. pylori isolates was analyzed by PCR. The antibiotic susceptibility of the isolates to amoxicillin, clarithromycin, levofloxacin, metronidazole, rifampicin and tetracycline was analyzed using the gradient method (E-test). Histopathologically, the presence of H. pylori was detected by immunohistochemical staining with anti-H. pylori antibodies. Results: Bacteriological analysis revealed that 70 (28.57%) of 245 samples were positive for H. pylori, with 71 H. pylori isolates obtained from 70 samples (two isolates from one sample). Molecular analysis showed 81 (33.06%) samples to be positive, while histopathological examination revealed that 76 (31.02%) were positive for H. pylori. Immunohistochemical staining showed that 64 (79%) of the 81 PCR-positive samples were positive. Antibiotic susceptibility testing showed resistance to metronidazole in 55 (77.46%) of 71 isolates, 19 (26.76%) to clarithromycin, 10 (14.08%) to levofloxacin and 4 (5.63%) to rifampicin. All isolates were sensitive to amoxicillin and tetracycline. Conclusions: The data obtained are of great epidemiological importance. In addition, the results of antibiotic susceptibility test may help to select appropriate antibiotics for the treatment of the disease.