Growth Hormone Gene Polymorphism in Four Cattle Breeds in Turkey

Korkmaz Agaoglu O., AKYÜZ B.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.19, no.3, pp.419-422, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 3
  • Publication Date: 2013
  • Doi Number: 10.9775/kvfd.2012.7961
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.419-422
  • Keywords: Growth Hormone, Cattle, Zavot, Polymorphism, FRAGMENT LENGTH POLYMORPHISM, MILK-PRODUCTION TRAITS, EAST ANATOLIAN RED, BOVINE SOMATOTROPIN, ALUI POLYMORPHISM, PROLACTIN GENES, BROWN SWISS, ASSOCIATION, HOLSTEIN, YIELD
  • Erciyes University Affiliated: Yes

Abstract

This study was conducted to determine DNA-polymorphism of a AluI RFLP at bovine growth hormone (bGH) gene in Zavot (n=48), East Anatolian Red (n=40), Simmental (n=94) and Brown Swiss (n=64) cattle breeds. A total of 246 cattle were genotyped for the bGH-AluI polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the study, two alleles (L and V) and three genotypes (LL, VV and LV) were revealed after than digestion of amplification product with AluI restriction enzyme. Allelic frequencies for EAR, SIM, BS and Zavot breeds were determined as 0.775, 0.734, 0.781 and 0.760 respectively for L allele; 0.225, 0.266, 0.219 and 0.240 respectively for V allele. Otherwise, genotypic frequencies were 0.65, 0.57, 0.63 and 0.63 for LL, 0.10, 0.11, 0.06 and 0.10 for VV, and 0.25, 0.32, 0.31 and 0.27 for LV respectively. A significant deviation from Hardy-Weinberg equilibrium was not observed in the investigated breeds. As a result, this study provided information on the polymorphism of bGH in four cattle breeds. Additionally, this study reported the existence of a genetic polymorphism at bGH gene in Zavot cattle breed for the first time.