Development and Evaluation of Europium-Based Quantitative Lateral Flow Immunoassay for the Chronic Kidney Disease Marker Cystatin-C
Journal of Fluorescence, vol.32, pp.419-426, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 32
- Publication Date: 2022
- Doi Number: 10.1007/s10895-021-02886-y
- Journal Name: Journal of Fluorescence
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chimica, Compendex, EMBASE, MEDLINE
- Page Numbers: pp.419-426
- Keywords: Fluorescent quantitative lateral flow assay, Europium nanoparticles, Sandwich assay, Time-resolved fluorescent
- Open Archive Collection: AVESIS Open Access Collection
- Erciyes University Affiliated: Yes
Abstract
This study aimed to establish a Europium label time-resolved fluorescence immunoassay (TRFIA) to detect the chronic kidney disease (CKD) biomarker Cystatin-C. An Europium based Time resolved fluorescence immunoassay was developed to detect the concentration of Cystatin-C in a urine sample to increase the sensitivity with captured anti-Cystatin-C antibodies immobilized on nitrocellulose membrane and then bonded with detection anti-Cystatin-C labelled with CM-EU, followed by fluorescence measurement using time-resolved fluorometry in 15 min. The performance of this TRFIA was evaluated using the clinical urine serum and compared with the ELISA assays. The linear calibration range was 0.015-32 mu g/ml, and the limit of detection (LOD) quantified was 0.0001 mu g/ml. This current work has improved the LOD of our previous work from 0.013 mu g/ml to 0.001 mu g/ml. These results indicated that the CM-EU nanoparticle-based LFIA is rapid, more sensitive, reliable, and reproducible for point-of-care testing of Cys-C concentrations in urine.