Development and Evaluation of Europium-Based Quantitative Lateral Flow Immunoassay for the Chronic Kidney Disease Marker Cystatin-C

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Natarajan S., Saatçi E., Joseph J.

Journal of Fluorescence, vol.32, pp.419-426, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32
  • Publication Date: 2022
  • Doi Number: 10.1007/s10895-021-02886-y
  • Journal Name: Journal of Fluorescence
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chimica, Compendex, EMBASE, MEDLINE
  • Page Numbers: pp.419-426
  • Keywords: Fluorescent quantitative lateral flow assay, Europium nanoparticles, Sandwich assay, Time-resolved fluorescent
  • Erciyes University Affiliated: Yes

Abstract

This study aimed to establish a Europium label time-resolved fluorescence immunoassay (TRFIA) to detect the chronic kidney disease (CKD) biomarker Cystatin-C. An Europium based Time resolved fluorescence immunoassay was developed to detect the concentration of Cystatin-C in a urine sample to increase the sensitivity with captured anti-Cystatin-C antibodies immobilized on nitrocellulose membrane and then bonded with detection anti-Cystatin-C labelled with CM-EU, followed by fluorescence measurement using time-resolved fluorometry in 15 min. The performance of this TRFIA was evaluated using the clinical urine serum and compared with the ELISA assays. The linear calibration range was 0.015-32 mu g/ml, and the limit of detection (LOD) quantified was 0.0001 mu g/ml. This current work has improved the LOD of our previous work from 0.013 mu g/ml to 0.001 mu g/ml. These results indicated that the CM-EU nanoparticle-based LFIA is rapid, more sensitive, reliable, and reproducible for point-of-care testing of Cys-C concentrations in urine.