Ghrelin was isolated as an endogenous ligand for the GH secretagogue receptor from rat stomach. Ghrelin plays an important physiological role in insulin secretion, glucose metabolism, and cardiovascular system. In this study, the focus was on relative ghrelin expression in the heart of long-term diabetic rats. A total of 36 male Wistar albino rats were divided into four groups: control, 1-, 2-, and 3-month diabetic rats. Diabetes was induced with streptozotocin (STZ; 40 mg/kg i.p.). The rats were decapitated under ketamine anesthesia, and their heart tissues were removed. Tissue ghrelin expressions were then compared. After diabetes for 1 month, the expression of ghrelin observed similar to control rats. However, the ghrelin expression increased in 2 and 3-month diabetic rats compared to the control. In conclusion, increased ghrelin expressions in 2- and 3-month diabetic rats may be protecting them from diabetic cardiomyopathy.