Amebicidal effects of fenugreek (Trigonella foenum-graecum) against Acanthamoeba cysts

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Kaya Y., Baldemir A., Karaman U., ILDIZ N., Arici Y. K., Kacmaz G., ...Daha Fazla

FOOD SCIENCE & NUTRITION, cilt.7, sa.2, ss.563-571, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/fsn3.849
  • Dergi Adı: FOOD SCIENCE & NUTRITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.563-571
  • Anahtar Kelimeler: Acanthamoeba castellanii, amebicidal effect, fenugreek, Trigonella foenum-graecum
  • Erciyes Üniversitesi Adresli: Evet

Özet

Trigonella foenum-graecum L. (TF) is known to the public as a chest emollient, mucous expectorant, laxative and is used to prevent maturation of boils and diabetes since ancient times. In this study, we aimed to determine the amebicidal effects against Acanthamoeba cysts. Plant extracts were prepared at concentrations of 1, 2, 4, 8, 16, and 32 mg/ml and were placed in a hemocytometer with cell counts 22 x 10(6) cell/ml. The fatty acid profiles of TF seeds were determined. Standard Acanthamoeba cysts were added and incubated at 25 degrees C. The viability of the parasite was checked and recorded at hours 3, 24, 48, 72, 96, and 102. The values of lethal concentration doses (LD50 and LD90) were calculated using probit analysis. This study revealed that T. foenum-graecum prevented proliferation of the parasite at certain times. However, further for in vivo and controlled experimental studies are needed in order to find out how to use this plant as medication.