Rapid and enhanced germination at low temperature of alfalfa and white clover seeds following osmotic priming


Tiryaki I., Kizilsimsek M., Kaplan M.

TROPICAL GRASSLANDS, vol.43, pp.171-177, 2009 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 43
  • Publication Date: 2009
  • Title of Journal : TROPICAL GRASSLANDS
  • Page Numbers: pp.171-177

Abstract

Seeds of alfalfa (Medicago sativa) and white
clover (Trifolium repens) were primed in 3
priming solutions [2% NaCl, 2% KNO3 and 300
g/L polyethylene glycol (PEG)] for 2 days at
20°C and their subsequent germination compared
with that of untreated seed. Four replications
of 50 seeds for germination and 25 seeds for
emergence were arranged in a 2-factor factorial
completely randomised design. All priming treatments
significantly (P<0.05) advanced germination
rate and percentage of white clover at 15°C,
but produced minimal or negative effects on
alfalfa. Priming with PEG significantly (P<0.05)
reduced time to 50% of final germination percentage
(G50 = 4.2 days) of white clover in comparison
with control seeds (G50 = 6.5 days). The
results indicated that priming seeds in KNO3 for
2 days at 20°C could improve germination rate
and germination percentage of white clover at
15oC. Possible reasons for the differing responses
in the two species are discussed.

Seeds of alfalfa (Medicago sativa) and white clover (Trifolium repens) were primed in 3 priming solutions [2% NaCl, 2% KNO(3) and 300 g/L polyethylene glycol (PEG)] for 2 days at 20 degrees C and their subsequent germination compared with that of untreated seed. Four replications of 50 seeds for germination and 25 seeds for emergence were arranged in a 2-factor factorial completely randomised design. All priming treatments significantly (P<0.05) advanced germination rate and percentage of white clover at 15 degrees C, but produced minimal or negative effects on alfalfa. Priming with PEG significantly (P<0.05) reduced time to 50% of final germination percentage (G(50) = 4.2 days) of white clover in comparison with control seeds (G(50) = 6.5 days). The results indicated that priming seeds in KNO(3) for 2 days at 20 degrees C could improve germination rate and germination percentage of white clover at 15 degrees C. Possible reasons for the differing responses in the two species are discussed.