Research Information System
Applied Ecology And Environmental Research, vol.18, no.1, pp.1611-1620, 2020 (SCI-Expanded)
Myrtle is known to be an important aromatic and medicinal plant species. Myrtle plant is
consumed mainly as spice having several uses in the perfumery, food and pharmaceutical industries. It is
also widely used for ornamental purposes such as fencing, roadsides, and green cutting. Plant tissue
culture techniques are an essential tool to micropropagate for the plant producer. TIS (Temporary
immersion systems) is an alternative plant tissue culture technique for micropropagation mechanism in
plant species. A new culture vessel named PlantForm TIS bioreactor has been recently developed. In the
present study, we aimed to evaluate and compare a new type of TIS bioreactors with agar media for
myrtle. MS (Murashige and Skoog) medium supplemented with 1 mg l-1 BAP (benzylaminopurine) was
used for multiplication, with 1 mg l-1
IBA (indole-3-butyric acid) for rooting. Two different immersion
times (4 h and 8 h) were compared with the solid agar medium. Plantlets growing in PlantForm systems
performed better than those in the solid medium. All data recorded at micropropagation and rooting
treatments were much more successful. In conclusion, PlantForm TIS bioreactor presents a valid
alternative to traditional in vitro micropropagation and rooting systems, resulting in a reduction of cost,
labor, and time for the mass propagation