Aptamer-Nanoparticle Assembly for Logic-Based Detection

SHUKOOR M. I., ALTMAN M. O., HAN D., BAYRAC A. T., Ocsoy I., ZHU Z., ...More

ACS APPLIED MATERIALS & INTERFACES, vol.4, no.6, pp.3007-3011, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 6
  • Publication Date: 2012
  • Doi Number: 10.1021/am300374q
  • Journal Name: ACS APPLIED MATERIALS & INTERFACES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3007-3011
  • Keywords: Boolean logic-based detection, aptamer, gold nanoparticle, nanoparticle, PDGF, VEGF, ULTRASENSITIVE DETECTION, COLORIMETRIC DETECTION, OPTICAL-PROPERTIES, MOLECULAR BEACONS, GOLD, GATES, VEGF, POLYNUCLEOTIDES, EXPRESSION, ADENOSINE
  • Erciyes University Affiliated: No

Abstract

In this work, gold nanoparticles perform Boolean logic operations in response to two proangiogenic targets important in cancer diagnosis and treatment: PDGF and VEGF. In the absence of protein target, gold nanoparticles are initially dispersed as a red solution; the addition of target proteins causes nanoparticle aggregation, turning the solution blue, as well as the release of dye-labeled aptamer probes, which causes an increase in fluorescence. These outputs constitute an AND or OR gate for simultaneous protein detection. We believe this logic-gate-based detection system will become the basis for novel rapid, cheap, and reliable sensors for diagnostic applications.