Aptamer-Nanoparticle Assembly for Logic-Based Detection

SHUKOOR M. I., ALTMAN M. O., HAN D., BAYRAC A. T., Ocsoy I., ZHU Z., ...Daha Fazla

ACS APPLIED MATERIALS & INTERFACES, cilt.4, sa.6, ss.3007-3011, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1021/am300374q
  • Dergi Adı: ACS APPLIED MATERIALS & INTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3007-3011
  • Anahtar Kelimeler: Boolean logic-based detection, aptamer, gold nanoparticle, nanoparticle, PDGF, VEGF, ULTRASENSITIVE DETECTION, COLORIMETRIC DETECTION, OPTICAL-PROPERTIES, MOLECULAR BEACONS, GOLD, GATES, VEGF, POLYNUCLEOTIDES, EXPRESSION, ADENOSINE
  • Erciyes Üniversitesi Adresli: Hayır

Özet

In this work, gold nanoparticles perform Boolean logic operations in response to two proangiogenic targets important in cancer diagnosis and treatment: PDGF and VEGF. In the absence of protein target, gold nanoparticles are initially dispersed as a red solution; the addition of target proteins causes nanoparticle aggregation, turning the solution blue, as well as the release of dye-labeled aptamer probes, which causes an increase in fluorescence. These outputs constitute an AND or OR gate for simultaneous protein detection. We believe this logic-gate-based detection system will become the basis for novel rapid, cheap, and reliable sensors for diagnostic applications.