DGAT1, CAST and IGF-I Gene Polymorphisms in Akkaraman Lambs and Their Effects on Live Weights up to Weaning Age

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BAYRAM D., AKYÜZ B., ARSLAN K., Ozdemir F., AKSEL E. G., ÇINAR M. U.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.25, sa.1, ss.9-15, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.9775/kvfd.2018.20055
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.9-15
  • Anahtar Kelimeler: Birth weight, DGAT1, Polymorphism, RFLP, Sheep, OVINE CALPASTATIN GENE, SINGLE NUCLEOTIDE POLYMORPHISM, QUANTITATIVE TRAIT LOCI, GROWTH TRAITS, CARCASS TRAITS, BIRTH-WEIGHT, SHEEP BREEDS, PCR-RFLP, ASSOCIATION, SURVIVAL
  • Erciyes Üniversitesi Adresli: Evet

Özet

Early live weight in sheep is important for lamb survival and average weight gain until slaughter. The aim of the present study was to investigate association between CAST-Mspl, DGAT1-Alul and IGF-1-Bsp143II polymorphisms and early live weights between birth and weaning age in Akkaraman lambs. A total of 374 lambs were genotyped for CAST-Mspl, DGAT1-Alul and IGF-1-Bsp143II polymorphisms by the polymerase chain reaction and restriction length polymorphism (PCR-RFLP) method. The results of PCR-RFLP analysis showed that the SNPs had three genotypes of CAST-Mspl polymorphism, two genotypes of DGAT1-Alul polymorphism and one genotype of IGF-1-Bsp143II polymorphism of these, CAST-Mspl-MM, DGAT1-Alul-CC and IGF-1-Bsp143II-BB were the predominant genotypes in the Akkaraman sheep breed. The result of Chi-square analysis indicated that the Akkaraman sheep breed was in Hardy-Weinberg equilibrium for the investigated polymorphic genes. At the DGAT1 locus, the CT genotype showed significantly heavier birth weight (P=0.044) compared to CC genotype. CAST gene did not show any association for the investigated traits. The results of this study demonstrate that the CT genotype had a positive effect on birth weight in Akkaraman sheep. We concluded that further investigations are needed in DGAT1-Alul polymorphism and live weight at different ages in sheep.