Essay on the nucleoli survey by the alpha- and beta-satellite DNA probes of the acrocentric chromosomes in mitogen-stimulated human lymphocytes


Demirtaş H. , Candemir Z. , Cucer N. , Imamoglu N. N. , Donmez H. , Bökesoy I.

ANNALES DE GENETIQUE, vol.43, no.2, pp.61-68, 2000 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 2
  • Publication Date: 2000
  • Doi Number: 10.1016/s0003-3995(00)01014-5
  • Title of Journal : ANNALES DE GENETIQUE
  • Page Numbers: pp.61-68

Abstract

The two constitutive heterochromatin (alpha- and beta-satellite DNA) probes of human acrocentric chromosomes were essayed separately to label the nucleoli in the phytohemagglutinin (PHA)-stimulated human lymphocytes. Fluorescent in situ hybridisation (FISH) results have shown that: a) whole (100 %) signal-nucleoli overlapping was obtained with both heterochromatin probes in maximally activated nuclei (MANs); b) partial overlapping was observed in non activated or slightly activated nuclei; c) random signal-nucleolus overlapping (background level) was found to be similar to 6 % by the NOR-irrelevant euchromatic probe (D5S23); d) Yq-nucleolus association in the MANs was found to be similar to 97 % without the subtraction, of the background level. We concluded that: a) acrocentric alpha- or beta-satellite DNA probes may be used as nucleolar markers only in the MANs and not in slightly activated or non-activated nuclei; b) the distances between rDNA loci and alpha-/beta-satellite DNA on human acrocentrics are short enough to permit their observation on the same nucleolus. (C) 2000 Editions scientifiques et medicales Elsevier SAS.