Effects of Low and High Maternal Protein Intake on Fetal Skeletal Muscle miRNAome in Sheep

AKYÜZ B., Sohel M. M. H., KONCA Y., ARSLAN K., GÜRBULAK K., ABAY M., ...Daha Fazla

ANIMALS, cilt.14, sa.11, 2024 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 11
  • Basım Tarihi: 2024
  • Doi Numarası: 10.3390/ani14111594
  • Dergi Adı: ANIMALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Erciyes Üniversitesi Adresli: Evet

Özet

Simple Summary: Prenatal maternal feeding is critical for fetal development. miRNAs may impart unique metabolic and immunologic effects on developing fetuses. The fetal muscle miRNAome was found to be altered via maternal protein intake levels. oar-miR-3957-5p and miR-329a-3p were up-regulated in low- and standard-protein diets vs. high-protein diets. Many of the putative miRNA target genes and genetic pathways identified were involved in known muscle disease and immune function. Prenatal maternal feeding plays an important role in fetal development and has the potential to induce long-lasting epigenetic modifications. MicroRNAs (miRNAs) are non-coding, single-stranded RNAs that serve as one epigenetic mechanism. Though miRNAs have crucial roles in fetal programming, growth, and development, there is limited data regarding the maternal diet and miRNA expression in sheep. Therefore, we analyzed high and low maternal dietary protein for miRNA expression in fetal longissimus dorsi. Pregnant ewes were fed an isoenergetic high-protein (HP, 160-270 g/day), low-protein (LP, 73-112 g/day), or standard-protein diet (SP, 119-198 g/day) during pregnancy. miRNA expression profiles were evaluated using the Affymetrix GeneChip miRNA 4.0 Array. Twelve up-regulated, differentially expressed miRNAs (DE miRNAs) were identified which are targeting 65 genes. The oar-3957-5p miRNA was highly up-regulated in the LP and SP compared to the HP. Previous transcriptome analysis identified that integrin and non-receptor protein tyrosine phosphatase genes targeted by miRNAs were detected in the current experiment. A total of 28 GO terms and 10 pathway-based gene sets were significantly (padj < 0.05) enriched in the target genes. Most genes targeted by the identified miRNAs are involved in immune and muscle disease pathways. Our study demonstrated that dietary protein intake during pregnancy affected fetal skeletal muscle epigenetics via miRNA expression.