© 2018 Inst. Nat. Cercetare-Dezvoltare Text. Pielarie. All rights reserved.Fashion and color are the foreground of the case in the first stage of the selection of textile products. That is why coloring in textiles has a great appeal. In particular, natural dyeing, which has caught up with new trends in recent times, has gained importance in this regard. In this study, pine cones were used in dyeing of wool fabrics with the help of five different mordanting agent and also mordant-free dyeings were performed too. Besides, wool fabrics dyed with pine cones have been evaluated in terms of light and washing fastnesses. It was found that different colors and shades can be obtained with the use of different mordanting agents and finally it was observed that pine cones can be used as a natural dye source. Moreover the antibacterial efficiencies of the dyed samples were investigated to see the effect of the used natural dye source and the mordanting agents. It was seen that the dyed samples have different antibacterial properties depending on the used mordanting agent and the bacteria tested on.