DNA aptamer functionalized gold nanostructures for molecular recognition and photothermal inactivation of methicillin-Resistant Staphylococcus aureus

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Atıf İçin Kopyala

ÖÇSOY I., Yusufbeyoğlu S., YILMAZ V., MCLAMORE E. S., ILDIZ N., ÜLGEN A.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.159, ss.16-22, 2017 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 159
• Basım Tarihi: 2017
• Doi Numarası: 10.1016/j.colsurfb.2017.07.056
• Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.16-22
• Anahtar Kelimeler: DNA aptamer, Nanostructures, Photothermal inactivation, Methicillin-Resistant staphylococcus aureus, HIGHLY SENSITIVE DETECTION, GRAPHENE OXIDE, NANOPARTICLES, ANTIBACTERIAL, ANTIBODIES, MECHANISM, NANORODS, THERAPY, PROTEIN, DESIGN
• Erciyes Üniversitesi Adresli: Evet

Özet

In this work, we report the development of DNA aptamer-functionalized gold nanoparticles (Apt@Au NPs) and gold nanorods (Apt@Au NRs) for inactivation of Methicillin-resistant Staphylococcus aureus (MRSA) with targeted photothermal therapy (PIT). Although both Apt@Au NPs and Apt@Au NRs specifically bind to MRSA cells, Apt@Au NPs and Apt@Au NRs inactivated similar to 5% and over 95% of the cells,respectively through PTT. This difference in inactivation was based on the relatively high longitudinal absorption of near infrared (NIR) radiation and strong photothermal conversion capability for the Apt@Au NRs compared to the Apt@Au NPs. The Au NRs served as a nanoplatform for the loading of thiolated aptamer and also provided multivalent effects for increasing binding strength and affinity to MRSA. Our results indicate that the type of aptamer and the degree of multivalent effect(s) are important factors for MRSA inactivation efficiency in PTT. We show that the Apt@Au NRs are a very effective and promising nanosystem for specific cell recognition and in vitro PTT. (C) 2017 Elsevier B.V. All rights reserved.