Acta Horticulturae, no.588, pp.293-298, 2002 (Journal Indexed in SCI Expanded)
This study was conducted to determine the most efficient in vivo method and conditions for dihaplodization of haploid melon (Cucumis melo L.) plants. Tested techniques were (i) colchicine application to lateral buds by medicine dropper, (ii) colchicine containing agar, (ii) colchicine imbibed cotton and (iii) shoot tip immersing in colchicine solutions. After applications, ploidy level of plants observed morphologically (leaf and flower dimension, presence of pollen) and measured by flow cytometry. The results showed that among the in vivo duplication method, shoot tip immersing into colchicine solutions was the most successful techniques. Dihaplodzation efficiency by shoot tip immersing was about % 91.67. Duplication rate was for times more than application by cotton, 12 to 30 times more than application by agar. Colhicine treatment by medicine dropper was not found an efficient technique in chromosomes duplication.