Intracellular biosynthesis of PbS quantum dots usingPseudomonas aeruginosaATCC 27853: evaluation of antibacterial effects and DNA cleavage activities


Ocal N., CEYLAN A. , DUMAN F.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.36, no.10, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 10
  • Publication Date: 2020
  • Doi Number: 10.1007/s11274-020-02917-z
  • Title of Journal : WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Keywords: Quantum dots, PbS, Biosynthesis, Antimicrobial activity, DNA cleavage, LEAD SULFIDE NANOPARTICLES, MICROBIAL SYNTHESIS, RESOLUTION, DELIVERY

Abstract

Bacterial biosynthesis of quantum dots (QDs) offers a green alternative for the production of nanomaterials with superior properties, such as tunable size dependent emission spectra and a long fluorescence lifetime. In this study, we have achieved intracellular production of PbS QDs usingPseudomonas aeruginosaATCC 27853. The characterization of these PbS QDS was performed by different techniques, such as Ultraviolet-visible (UV-Vis) spectroscopy, photoluminescence (PL), X-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX) and particle size analysis (DLS). The obtained results confirmed the synthesis of PbS QDs. The PbS QDs showed absorption peaks at 1088 nm (ultraviolet-visible spectrometry) and a luminescence peak at 1572 nm. In addition, the intracellular biosynthesized PbS QDs showed a particle size in the range 3.47-11.45 nm, which is consistent with DLS and sphere-shaped nanocrystals with good crystallinity and a cubic cristalline structure including pure Pb and S elements. Biosynthesized PbS QDs showed antibacterial activity againstProteus mirabilisATCC 25933 andEscherichia coliATCC 25922 from Gram (-) bacteria andBacillus cereusNRLL B-3008 andMicrococcus luteusATCC 10240 from Gram (+) bacteria. Besides, these current results obtained from the cleavage studies revealed that PbS QDs do not show DNA cleavage activity. Consequently, the microorganism-based intracellular method allows an economic and environmentally friendly way to obtain PbS QDs with superior optical properties and they have a potential to be used in healthcare applications.