Background: The present study aims to investigate the effects of calcium fructoborate on testicular DNA damage and testicular tissue biochemical markers and serum testosterone levels after cadmium chloride administration. Materials and Methods: 28 Wistar albino rats (200-220 g) in the study were divided into 4 groups with an equal number. These groups are; Control group (No chemicals applied), calcium fructoborate (100 mg) group, Cadmium chloride (200 mg/L) + calcium fructoborate (100 mg), Cadmium chloride (200 mg/L) group. The study lasted 28 days and both chemicals were applied daily with oral gavage. Results: While 8-hydroxy-2-deoxyguanosine (8-OHdG) expression was moderate in the cadmium chloride + calcium fructoborate group, the expression in the cadmium chloride group was severe. In the cadmium chloride group, testicular tissue glutathione (GSH) and 8-OHdG levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and serum testosterone levels were significantly decreased compared to other groups. It is determined that the reversal of the change in the parameters listed in the calcium fructoborate group. Conclusion: The reversal of the change in the parameters listed in the calcium fructoborate group indicates the positive strength of the present chemical. It is our suggestion to transfer calcium fructoborate into life practice by conducting further clinical studies and evaluating different parameters.