EFFECTS OF RESVERATROL AND THYMOQUINONE ON DOXORUBICIN-INDUCED RAT TESTICULAR DAMAGE: HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL EVALUATIONS


Öztürk A. E., Ateş M. B., Özdemir Ö., Bucak M. N.

Ist INTERNATIONAL 10th NATIONAL REPRODUCTION AND ARTIFICIAL INSEMINATION SCIENCE CONGRESS, Konya, Turkey, 28 September - 01 October 2022, pp.109-110

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.109-110
  • Erciyes University Affiliated: Yes

Abstract

In the presented study, harmful effects of doxorubicin on rat testicular tissue were determined and the protectiveness of thymoquinone and resveratrol have been studied. A total number of 80 male Albino Wistar rats, including 10 groups and 8 male rats in each group, were used in the study. Experimental groups were designed as; control, 15 mg/kg doxorubicin (D), 5mg/kg thymoquinone (T5), 20mg/kg thymoquinone (T20), 5mg/kg resveratrol (R5), 20mg/kg resveratrol (R20), D+T5, D+T20, D+R5 and D+R20. For the sham effect, physiological saline was administered to the control group every other day by oral gavage. In addition to the applications in the control group, D group recieved doxorubicin at a dose of 15 mg/kg intraperitoneally (IP) on the 10th day. At the end of the 21th day, rats euthanised by cervical dislocation method under the anesthesia then samples collected for pathologic measurements immediately. When the relative testicular weights were evaluated, the group containing doxorubicin (0.3672) caused a significant decrease compared to the control group (0.4371%). D+T5 (0.4559%), D+T20 (0.4972%), D+R5 (0.4497%) and D+R20 (0.4845 g) were able to prevent testicular weight loss caused by doxorubicin. Germinative cell layer thickness in animals treated with doxorubicine (32.9 µm) decreased considerably compared to the control (59.91 µm). In the combined groups, D+R5 (58.85 µm) gave the most successful result. When the diameters of the tubulus seminiferus contortus were considered, it was found that while the average tubulus diameter of the control group was measured at 308.6 m, it was measured at 232.33 m in the doxorubicine Although the D+T5 and D+T20 treatments offered some protection against doxorubicin’s harmful effects, their tubulus diameter values could not match those of the control group. D + R5 (292.59 µm) and D+R20 (292.55 µm) groups preventing the damaging effect of doxorubicin in terms of tubular diameter and brought it closer to the average value of the control (p0,05). When the results of the immunohistochemical analyzes were evaluated, the Bax value was 4.19 in the control group, while it was quite high in the doxorubicin group (12.37 Although the amount of Bax in all thymoquinone and resveratrol groups used in combination with doxorubicin was lower than the group in which only doxorubicin was administered, the D+T5 group gave the best result with 5.75. When the Bcl-2 results were evaluated, the Bcl-2 value of the doxorubicin group (2.86) was significantly lower than all other groups. While this value was 17.25 in the control group, D+T5 (18.57), D+T20 (16.36) and D+R20 (18.98) gave similar results to that of control (p>0,05) and gave higher Bcl-2 scores than doxorubicin administrated group (p<0,05).