The Investigation of Fasciolosis in Sheep by Coproantigen ELISA and Sedimentation-Zinc Sulphate Flotation Technique Around Zara Region (Turkey)


Karapinar A., Yildirim A., Biskin Z., Duzlu Ö., Inci A.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.18, 2012 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18
  • Basım Tarihi: 2012
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Erciyes Üniversitesi Adresli: Evet

Özet

This study was carried out on totally 201 sheep in Zara of Sivas between September 2008 and June 2009 to determine the prevalence of fasciolosis. Collected feces samples were examined by sedimentation-zinc sulphate flotation technique. Modified McMaster sedimentation technique was applied to determine the EPG values. Copro antigens were investigated by copro-antigen ELISA. The prevalence of fasciolosis was determined as 3.48% and 5.97% according to faecal sedimentation-flotation and coproantigen ELISA, respectively. The mean number of Fasciola spp. egg per gram of feces (EPG) in infected sheep was 69.8 +/- 31.2. The prevalence of other parasites such as Ostertagia spp., Nematodirus spp., Trichostrongylus spp., Eimeria spp., Moniezia spp., Trichuris ovis, Paramphistomum spp. and Dicrocoelium dentriticum was determined as 43.2%, 41.2%, 38.3%, 17.4%, 1.49%, 0.99%, 0.49% and 0.49%, respectively. Fasciolosis was observed higher in 4-6 age group (8.3%) than 1-3 age group (3.7%), however, the differences between age groups were not found significant. The prevalence of fasciolosis in female and male sheep was found as 8.5% and 1.7%, respectively and this difference was not found significant. Although fasciolosis prevalence was found to be higher in Akkaraman breed (8.0%) than Kangal type (2.0%), this difference was also found insignificant. In conclusion, the prevalence of fasciolosis in sheep in Zara district of Sivas was revealed by specific coproantigen ELISA with this study. These results provide a contribution for planning future research and control strategies against fasciolosis in the research area.