The plasma lipid peroxidation intensity (MDA) and chain-breaking antioxidant concentrations in the cows with clinic or subclinic mastitis

Kizil O., Akar Y., Saat N., Kizil M., Yukseu M.

REVUE DE MEDECINE VETERINAIRE, vol.158, no.11, pp.529-533, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 158 Issue: 11
  • Publication Date: 2007
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.529-533
  • Erciyes University Affiliated: No


The aim of this study was to determine lipid peroxidation intensity by measurement of plasma malonedialdehyde (MDA) concentrations and plasma chain-breaking antioxidant concentrations such as reduced glutathione (GSH), vitamin A, E, C and beta-carotene in the cows with and without mastitis. The study was conducted on 30 Holstein cows which were divided into 3 equal groups on the basis of clinical examination and California Mastitis Test (CMT): in the group CM, clinical mastitis was evidenced; in the group SCM, mastitis was subclinical, and the group C served as a control. The plasma lipid peroxidation index was measured according to the concentration of thiobarbituric acid reactive species (MDA), and GSH activity was measured spectrophotometrically using Ellman's reagent. Vitamin A, P-carotene and vitamin E values were spectrophotometrically determined, and vitamin C value was colorimetrically determined using a phosphotungstic acid method. The mean plasma GSH (p<0.01) and beta-carotene (p<0.001) concentrations were showed significant differences between the 3 groups, whereas mean plasma MDA and serum vitamin C concentrations significantly differed only between the C and CM groups (p<0.05). The decreased chain-breaking antioxidant concentrations and the enhanced concentrations of GSH and MDA suggest that an inflammatory-related oxidative stress occurs in the cows with clinical and subclinical mastitis.