Abiotic factors affecting in vitro pollen grain germination in sainfoin (Onobrychis viciifolia Scop.)


Sancak C., Cocu S. , Khawar K., Ozcan S.

PAKISTAN JOURNAL OF BOTANY, vol.35, no.4, pp.463-471, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 4
  • Publication Date: 2003
  • Title of Journal : PAKISTAN JOURNAL OF BOTANY
  • Page Numbers: pp.463-471

Abstract

A reliable in vitro method for germination of sainfoin pollen grains has been developed. Highest pollen germination rate of 96% with mean pollen tube length of 223.5 mum was obtained on medium containing of 100 g/l sucrose, 200 mg/l KNO3, 150 mg/l H3BO3, 150 mg/l MgSO4, 150 mg/l Ca(NO3)(2), 175 g/l PEG 4000 and stigma extract at pH 6.5.