Assessment of DNA damage and plasma catalase activity in healthy term hyperbilirubinemic infants receiving phototherapy

KARAKÜKCÜ Ç., Ustdal M., ÖZTÜRK A., BAŞKOL G., Saraymen R.

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, vol.680, pp.12-16, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 680
  • Publication Date: 2009
  • Doi Number: 10.1016/j.mrgentox.2009.07.016
  • Journal Name: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.12-16
  • Keywords: DNA damage, Hyperbilirubinemia, Phototheraphy, Comet assay, COMET ASSAY, NEONATAL HYPERBILIRUBINEMIA, OXIDATIVE STRESS, FOLLOW-UP, IN-VITRO, BILIRUBIN, ANTIOXIDANT, PRETERM, MECHANISM, ABSENCE
  • Erciyes University Affiliated: Yes

Abstract

Phototherapy (PT) is the most widely used form of treatment for unconjugated hyperbilirubinemia. One possible harmful consequence of PT is of a genetic nature. High levels of bilirubin may lead to oxidative damage in newborns: photochemical reactions may produce toxic photoproducts, probably peroxides. In order to investigate this hypothesis further under in vivo conditions, DNA strand-break frequency was examined by means of the comet assay in peripheral lymphocytes of icteric newborns undergoing PT treatment, and the levels of catalase, an antioxidant enzyme, were determined.