A simple, sensitive, effective and rapid liquid-phase microextraction (LPME) method based on deep eutectic solvent (DES) was developed for the preconcentration and determination of erythrosine in the drug, water and powdered fruit juice samples prior to its UV-VIS spectrophotometric determination. In the DES-LPME study, the analytical parameters affected on extraction efficiency of the analyte were optimized. Under the optimum experimental conditions, the limits of detection and limit of quantification were obtained as 0.53 mu g L-1 and 1.78 mu g L-1, respectively. The preconcentration factor was calculated as 100. Accuracy of the proposed microextraction method has been checked by analyzing the addition-recovery studies to in real samples and satisfactory results were obtained.