Akademik Veri Yönetim Sistemi
XIII International Scientific Agriculture Symposium “AGROSYM 2022”, Bosna-Hersek, 6 - 09 Ekim 2022, sa.125
In this study, Lipoteichoic acid (LTA, n=7, 50 μg/kg), Lipopolysaccharide (LPS, n=7, 20 μg/kg) and LTA-LPS mixture (n=7, 50 μg/kg + 20 μg/kg) and PBS (control, n=7, 250 μl) were administered via intranasal route to Akkaraman lambs. The differences in the expression of TLR2, TLR4, MyD88, TRAF6, TNF-α, IL-1ß, IL-6, IL-10, NF-κß and IFNγ genes using GAPDH and ß- actin as reference genes, and the relationship of genes with each other’s both in different and in the same time periods were determined with qRT-PCR in PBMCs isolated at the 24th hour and on the 7 th day after administration. The correlation coefficient and statistical significance between the normalized expression data of genes were examined by Spearman rho correlation analysis. Considering the obtained results at the 24 th hour; high and statistically significant (p<0.05) positive correlations were determined between TLR2-IL10, NF-κß, NF-κßIL-10 genes in the control group; between TLR2-TNF-α, IL-10, TLR4-MyD88, IL-1ß, MyD88-IL1ß, IFNγ-TRAF, NF-κß, TNF-α-IL-10 genes in the LTA group; between TLR2-TRAF6, TNF-α, IL-10, IL-10-TLR4, TRAF6, IL-1ß genes in the LPS group and between TLR2-IL-10, TLR4TRAF6 genes in the mixture group. On the 7 th day, high level of (p<0.05) positive correlations were determined between TLR2-TLR4, IL-1ß, IL-10, IFNγ, TLR4-IL10, TRAF6-NF-κß, TNF-α, IL1-ß-IL-10 genes in control group, between TLR2-TLR4, TNFA, IL-10, TLR4-MyD88, NF-κß, TNF-α, NF-κß, MyD88, TNF-α genes in the LTA group and between IL-10-IFNγ genes in the LPS group whereas a statistically significant high level of negative correlation (p<0.05) was determined between NF-κß-IL-1ß genes in the mixture group. Considering the correlation between time periods, moderate and high level of negative correlations (p<0.05) were observed between genes in all groups except the control group. According to the obtained results, the applied molecules activated different genes in different groups at the 24 th hour and the activity decreased on the 7 th day.