Immunolocalization of Vascular Endothelial Growth Factor Its Receptors flk1 fms flk1 KDR flt4 and Vascular Endothelial Growth Inhibitor in quail spleen Coturnix coturnix japonica during the Post Hatching Period

Sağsöz H., Liman N. , Alan E.

30th European Association of Veterinary Anatomists Congress, Cluj-Napoca, Romanya, 23 - 26 Temmuz 2014, cilt.43, ss.82

  • Cilt numarası: 43
  • Basıldığı Şehir: Cluj-Napoca
  • Basıldığı Ülke: Romanya
  • Sayfa Sayıları: ss.82


Introduction: The formation of blood vessels allows the

growth and functioning of organs and the vascular endothelial

growth factor (VEGF) and its receptors have been

implicated as key players in this process. The spleen is an

immunologic organ with a very rich vascular supply. In

the present study, the question of whether VEGF, its

receptors (flt1/fms, flk1/KDR and flt4) and vascular endothelial

growth inhibitor (VEGI) are expressed in the vascular

system and lymphoid tissue of quail spleen during

the post-hatching period was investigated using immunocytochemistry.

Methods: Thirty quails were obtained from the Safiye

Cikrikcioglu Vocational College, Erciyes University (Kayseri).

Five quails each, aged 1, 7, 14, 21, 28, and 60 days, were sacrificed

by means of ether anaesthesia and their spleens were

quickly removed for immunohistochemical analysis.

Results: Both, a membrane and cytoplasmic pattern of

staining for VEGI, VEGF and its receptors were observed

in some cell types of peri-ellipsoidal white pulp (PWP) and

periarterial lymphoid sheath (PALS), endothelial cells of

penicillar capillaries (Pc) and central arteries (CA) as well

as venous sinuses (S) of red pulp in the spleen throughout

the post-hatching period. Flt1/fms and Flk1/KDR immunoreactivities

in the Pc and CA and S were stronger than

those of VEGF, VEGI and flt4, particularly from hatching

to the 21th days post-hatching. Besides, VEGI immunoreactivity

in the Pc, some cell types of PWP,PALS and in S

were strong on days 1, 7, and 14, but negative on days 21,

28 and 60 of the post-hatching period. However, VEGF

and Flt4 immunoreactivities were weak in all components

of the spleen during post-hatching period.

Conclusions: Our findings show that the localizations of

VEGF, flt1/fms, flk1/KDR, flt4 and VEGI in structural

components of quail spleen are altered during the days of

the post-hatching period.

This study was approved by the Ethics Committee of

Erciyes University.