30th European Association of Veterinary Anatomists Congress, Cluj-Napoca, Romania, 23 - 26 July 2014, vol.43, pp.82
Introduction: The formation of blood vessels allows the
growth and functioning of organs and the vascular endothelial
growth factor (VEGF) and its receptors have been
implicated as key players in this process. The spleen is an
immunologic organ with a very rich vascular supply. In
the present study, the question of whether VEGF, its
receptors (flt1/fms, flk1/KDR and flt4) and vascular endothelial
growth inhibitor (VEGI) are expressed in the vascular
system and lymphoid tissue of quail spleen during
the post-hatching period was investigated using immunocytochemistry.
Methods: Thirty quails were obtained from the Safiye
Cikrikcioglu Vocational College, Erciyes University (Kayseri).
Five quails each, aged 1, 7, 14, 21, 28, and 60 days, were sacrificed
by means of ether anaesthesia and their spleens were
quickly removed for immunohistochemical analysis.
Results: Both, a membrane and cytoplasmic pattern of
staining for VEGI, VEGF and its receptors were observed
in some cell types of peri-ellipsoidal white pulp (PWP) and
periarterial lymphoid sheath (PALS), endothelial cells of
penicillar capillaries (Pc) and central arteries (CA) as well
as venous sinuses (S) of red pulp in the spleen throughout
the post-hatching period. Flt1/fms and Flk1/KDR immunoreactivities
in the Pc and CA and S were stronger than
those of VEGF, VEGI and flt4, particularly from hatching
to the 21th days post-hatching. Besides, VEGI immunoreactivity
in the Pc, some cell types of PWP,PALS and in S
were strong on days 1, 7, and 14, but negative on days 21,
28 and 60 of the post-hatching period. However, VEGF
and Flt4 immunoreactivities were weak in all components
of the spleen during post-hatching period.
Conclusions: Our findings show that the localizations of
VEGF, flt1/fms, flk1/KDR, flt4 and VEGI in structural
components of quail spleen are altered during the days of
the post-hatching period.
This study was approved by the Ethics Committee of