Development of molecular markers linked to QTL/genes controlling Zn efficiency


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PINAR H., Bulbul C., Simsek D., Shams M., MUTLU N., ERCİŞLİ S.

MOLECULAR BIOLOGY REPORTS, vol.49, no.6, pp.5273-5281, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 6
  • Publication Date: 2022
  • Doi Number: 10.1007/s11033-021-06736-9
  • Journal Name: MOLECULAR BIOLOGY REPORTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.5273-5281
  • Keywords: Pepper, Zn efficiency, Genetic mapping, SSR, SRAP, RAPD, QUANTITATIVE TRAIT LOCI, ZINC-DEFICIENCY, IDENTIFICATION, INHERITANCE, GROWTH, TOLERANCE, CROPS, YIELD
  • Erciyes University Affiliated: Yes

Abstract

Background Zinc (Zn) deficiency is a widespread problem in reducing the yield and quality of crop plants worldwide. It is important to utilize molecular markers linked to Zn efficiency to develop high Zn efficient cultivars in pepper (Capsicum annuum L.). Methods and results In present study, genetic map was constructed using a F-2 populations derived from C. annuum L. (Alata 21A) x C. frutescens L. (PI 281420) cross. The quantitative trait locus (QTLs) for Zn efficiency were mapped using F-2:3 population. A genetic map with 929.6 cM in length and 12 linkage groups were obtained using 62 markers (31 sequence-related amplified polymorphism (SRAP), 19 simple sequence repeat (SSR) and 11 random amplified polymorphic DNA (RAPD) markers). The 41 linked QTLs related with nine (9) Zn efficiency characters were mapped on linkage groups. Results suggest that selecting plants for tolerance to Zn deficiency are expected to be rather responsive among segregating populations for breeding and developing Zn efficient genotypes in pepper. Conclusions The molecular markers are expected to aid selection and expedite breeding peppers resistant to Zn deficiency in soils low for available Zn contents.