In the title molecule, C27H25N3O3S center dot H2O, the dihedral angle between the planes of the thienyl ring and the pendant p-tolyl group is 39.25 (6)degrees, while that between the pyridine ring and the pendant phenyl ring is 44.37 (6)degrees. In addition, there is a slight twist in the bicyclic core, with a dihedral angle of 2.39 (4)degrees between the thienyl and pyridine rings. The conformation of the carbamoyl moiety is partially determined by an intramolecular N-H center dot center dot center dot O hydrogen bond. In the crystal, complementary N-H center dot center dot center dot O hydrogen bonds form dimers which are then associated into chains parallel to the c axis through O-H center dot center dot center dot N hydrogen bonds involving the water molecule of crystallization. Electron density associated with an additional solvent molecule of partial occupancy and disordered about a twofold axis was removed with the SQUEEZE procedure in PLATON [Spek (2015). Acta Cryst. C71, 9-18]. The given chemical formula and other crystal data do not take into account the unknown solvent molecule(s).