This study was conducted to determine the effects of plant growth promoting bacteria (PGPR) and boron (Bio-B) on the plant freeze injury, antioxidant enzyme activity and fruit yield of grapevine cultivar. The experimental plot was acompletely randomized design with 5replicates. As atrial model Bio-B and control were used as fertilizer agent. Bio-B fertilizer has been applied in different ways as soil + foliar, soil and foliar application methods to grapevine plants. Data through 2 years trials results showed that the use of Bio-B significantly decreased freeze injury and increased antioxidant enzyme activity of grapevine leaf. The highest damage rate at control group occurred with -20 degrees C and 94.89% ratio. In the same temperature degree at grape plant, the damage ratio of the Bio-B application from soil decreased by 21.55%; the application from leaf by 25.53% and with the application from soil + leaf by 26.24%. In addition to this, compared with control, in the CAT, POD and SOD enzyme activities increases occurred with the ratios as 28.57%, 22.05% and 39.25%, respectively. Generally, as results of this study under field conditions, Bio-B fertilizer application decreased freeze injure and increased antioxidant enzyme activity of the grapevine plant.