Determination of Polycyclic Aromatic Hydrocarbons (PAHS) in Sewage Sludge and Compost by Dispersive Solid-Phase Microextraction (SPME) and Ultra-High Performance Liquid Chromatography (UHPLC) with Diode Array Detection (DAD)

ŞAHAN S., Sahin U., Jakubus M.

ANALYTICAL LETTERS, cilt.56, sa.5, ss.832-846, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/00032719.2022.2106237
  • Dergi Adı: ANALYTICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.832-846
  • Anahtar Kelimeler: Polycyclic aromatic hydrocarbons (PAHs), sewage sludge and compost, dispersive solid-phase microextraction (SPME), water purification, ultra-high performance liquid chromatography (UHPLC), MICROWAVE-ASSISTED EXTRACTION, ULTRASONICATION EXTRACTION, HPLC, SOIL, CLEANUP, SAMPLES, SPE
  • Erciyes Üniversitesi Adresli: Evet

Özet

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic that may degrade human health. Organic solvents are commonly used for the extraction of PAHs from solid samples and the analytes are determined chromatographically. This study describes the ultra-high performance iquid chromatography-diode array detection (UHPLC-DAD) determination of PAHs in sewage sludge and compost used as agricultural fertilizer. The PAHS may be transmitted to humans through the food chain. Analytes in the fertilizer were quantitatively extracted into the organic phase in water-dichloromethane-acetonitrile and determined liquid chromatographically. 16 PAHs were selectively extracted in a single clean-up step. For method development, the type and volume of organic solvent, pH of the water, and ultrasonic extraction time were optimized. For method validation, analytical figures of merit were obtained for the linearity, limit of detection (LOD), limit of quantification (LOQ), stability, intra- and inter-day precision, and accuracy. The developed and validated method was used for PAH determination in the fertilizers. Sample analyses were performed with 89-102% recovery values and 0.7-4.1% relative standard deviations.