Human feeder cell line for derivation and culture of hESc/hiPSc

McKay T. R., Camarasa M. V., Iskender B., Ye J., Bates N., Miller D., ...More

STEM CELL RESEARCH, vol.7, no.2, pp.154-162, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.1016/j.scr.2011.04.005
  • Journal Name: STEM CELL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.154-162
  • Erciyes University Affiliated: No

Abstract

We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use. (C) 2011 Elsevier B.V. All rights reserved.