The Effect of Trıs-Based Taxıfolın And Trehalose Extender On Post-Thawed Ram Semen Parameters


Bucak M. N. , Keskin N., Öztürk A. E. , Başpınar N., İli P.

BIALIC CONGRESS. INTERNATIONAL BIOLOGICAL, AGRICULTURAL LIFE SCIENCE CONGRESS, Lviv, Ukraine, 7 - 08 November 2019, pp.96

  • Publication Type: Conference Paper / Summary Text
  • City: Lviv
  • Country: Ukraine
  • Page Numbers: pp.96

Abstract

In this study, the effect of taxifolin and trehalose added tris based extender were investigated on freezebility of ram semen. Semen were collected from rams with helping of artificial vagina. Semen were pooled and were divided eight equal parts. Groups were performed as follows: glycerol 5% (G5), glycerol 5% + 10 μM taxifolin (G5T10), glycerol 5%+ 100 μM taxifolin (G5T100), glycerol 5% + 500 μM taxifolin (G5T500), glycerol 3% + 60 mM trehalose (G3TRE), glycerol 3% + 60 mM trehalose + 10 μM taxifolin (G3TRET10), glycerol 3% + 60 mM trehalose + 100 μM taxifolin (G3TRET100), glycerol 3% + 60 mM trehalose + 500 μM taxifolin (G3TRET500). Semen were equilibrated for 2 hours. After equilibration diluted semen froze in 0.25 ml French straws in liquid nitrogen. Straws were thawed for 25 second in a water bath. The levels of lipid peroxidation (LPO), glutatyon (GSH) and total antioxidant capacity (AOC) were assessed. When LPO levels were examined, it was determined that the level of G5 group was significantly higher than those of G5T500 and G3TRET100 groups (p <0.05). There were no significant difference between GSH and AOC for all groups.