Diagnosis of <i>Campylobacter</i> spp. Isolates and Their Antimicrobial Susceptibility Patterns

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Alobaidy H. A. K., Ayvaz A., Sağıroğlu P., Atalay M. A.

INDIAN JOURNAL OF MICROBIOLOGY, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s12088-024-01199-5
  • Dergi Adı: INDIAN JOURNAL OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database
  • Anahtar Kelimeler: Antibiotic resistance, Campylobacterspp, MALDITOF MS, QRDR, Quinolone
  • Erciyes Üniversitesi Adresli: Evet

Özet

This study aimed to reveal antibiotic resistance patterns and molecular characterization of quinolone resistance Campylobacter isolates in patients with diarrhea. Campylobacter spp. isolated from 35.33% of the total samples, most of which were from male patients aged 3 months to 10 years. Identifying isolates at the species level made in MALDI-TOF MS, 82.4% were C. jejuni, and 17.6% were C. coli. Respectively 94% (47/50), 58% (29/50), and 2% (1/50) resistance rates were determined for ciprofloxacin, tetracycline, and erythromycin. While C. jejuni isolates were more resistant to ciprofloxacin and tetracycline than C. coli, they showed no resistance to erythromycin. Quinolone resistance determining region (QRDR) were evaluated by mismatch amplification mutation test and all quinolone resistant strains gave positive results. One of the seven silent mutations identified was specific to this study, and two other novel mutations were also identified.