Membrane filtration of Sudan orange G on a cellulose acetate membrane filter for separation-preconcentration and spectrophotometric determination in water, chili powder, chili sauce and tomato sauce samples

AlOthman Z. A. , Unsal Y. E. , Habila M., Shabaka A., Tuzen M., SOYLAK M.

FOOD AND CHEMICAL TOXICOLOGY, vol.50, no.8, pp.2709-2713, 2012 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 50 Issue: 8
  • Publication Date: 2012
  • Doi Number: 10.1016/j.fct.2012.05.021
  • Page Numbers: pp.2709-2713


A simple membrane filtration procedure for separation-enrichment of Sudan orange G is presented. The method is based on the adsorption of Sudan orange G on a cellulose acetate filter and its elution from the membrane with 10 mL of ethanol. Sudan orange G in the eluent was determined by UV-visible spectrophotometry at 388 nm. The effect of analytical conditions, including pH, flow rates and eluent, sample volume, type of membrane for quantitative preconcentration and separation of Sudan orange G were examined. The influences of matrix components on Sudan orange G recoveries were studied. The preconcentration factor was 125. The detection limit was 4.9 mu g L-1. The relative standard deviation was 4.3%. The presented procedure was applied to chili powder, chili sauce, tomato sauce, powdered beverage and water samples. (C) 2012 Elsevier Ltd. All rights reserved.