Bacillus vallismortis and Bacillus mojavensis were loaded onto Amberlite XAD-4 resin and used for solid phase extraction (SPE) of uranium(VI). A quick and simple UV-Vis spectrophotometric method was used to determine U(VI) ion. The best experimental conditions were determined as being a pH of 5.0; a sample flow rate of 2.0 mL min(-1); 200.0 mg of biosorbent; 800 mg of Amberlite XAD-4, and 5.0 mL of 1 mol L-1 HCl as desorption solution for both immobilized bacteria. The preconcentration factors were achieved as 80 for both solid phase extractor. The developed methods were validated by applying to reference water and tea samples.