The protective role of melatonin against the effects of different doses of caffeine on the fetus


YİLMAZ S., GÖÇMEN A. Y., Arikan E. S., Akyuz E., Tokpinar A., NİSARİ M., ...Daha Fazla

CELLULAR AND MOLECULAR BIOLOGY, cilt.66, sa.5, ss.169-178, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66 Sayı: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.14715/cmb/2020.66.5.29
  • Dergi Adı: CELLULAR AND MOLECULAR BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.169-178
  • Anahtar Kelimeler: Rat, Caffeine, Ossification, Oxidative stress, Pregnancy, RENIN-ANGIOTENSIN SYSTEM, EHRLICH ASCITES, BONE TISSUE, RAT, METABOLISM, EXPRESSION, EXPOSURE, RECEPTOR, TUMOR, MOOD
  • Erciyes Üniversitesi Adresli: Evet

Özet

Skeletal system and some organs development changes in rat fetuses with 30 and 60 mg/kg caffeine and melatonin's (10 mg/kg) protective role against rat fetuses were investigated. Groups (n = 4) were formed as Control, LDC, HDC, LDC+melatonin, HDC+melatonin and melatonin. Fetuses were taken by cesarean section and stained using dual skeletal staining method and FESEM. TRAP and AP immune-reactivity concentrations were calculated. Oxidative stress and inflammatory markers were also measured by liver, bone and placenta samples. TNT-alpha, IL-1 beta, IL-6, VEGF-A, SOST and Fetuin-A levels were measured in tissue by using ELISA. TBARS, SOD, GSH, GSSG, TOS, TAS, measured by spectrophotometric assay method. The mRNA levels of Agtr2 gene expressed in placental tissues of control rats and in placental tissues of rats exposed to HDC, LDC, MEL, HDC+MEL, LDC+MEL were analyzed by Real-time PCR. The gene expressions of Agtr2 were significantly upregulated in the placentas exposed to HDC. MEL. HDC+MEL and LDC+MEL (P<0,001). No significant difference in samples of the LDC group (P>0,05). According to these data, caffeine used during pregnancy delayed ossification; melatonin, a powerful antioxidant, was found to eliminate this effect. Besides, changes in angiotensin receptor expression observed in response to a caffeine and melatonin exposure result from high dose and join effect.