Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc

Yuvalı D. , Narin İ.

Cumhuriyet Science Journal, vol.41, no.1, pp.239-244, 2020 (National Refreed University Journal)

  • Publication Type: Article / Article
  • Volume: 41 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.17776/csj.658063
  • Title of Journal : Cumhuriyet Science Journal
  • Page Numbers: pp.239-244


In this study, a simple, sensitive, precise, rapid and reversed-phase high performance liquid chromatographic method with ultraviolet detection for simultaneous determination of Sulfachloropyridazine and Trimethoprim is developed and applied commercial veterinary formulation for chromatographic separation, Thermoscientific Hypersil-C18, reverse phase column was used. Separation was done using acetonitrile: pH 3.0 buffer solution (30:70, v/v) at 0.8 ml min-1 flow rate. Analysis was done 272.0 nm at ultraviyolet detector. Under optimum chromatographic conditions, retention time of Sulfachloropyridazine and Trimethoprim were determined as 4.37 and 2.60 minute. The proposed method was linear in the range 1.0-100.0 μg ml-1 for Sulfachloropyridazine and 0.5-40.0 μg ml-1 for Trimethoprim. The Limit of detection of Sulfachloropyridazine and Trimethoprim are 0.05 μg ml-1 , 0.18 μg ml-1 , respectively. The method which is rapid, simple and does not require any separation step, has been successfully applied to assay of commercial dosage forms containing Sulfachloropyridazine and Trimethoprim