Research Information System
Cumhuriyet Science Journal, vol.41, no.1, pp.239-244, 2020 (Peer-Reviewed Journal)
In this study, a simple, sensitive, precise, rapid and reversed-phase high performance liquid
chromatographic method with ultraviolet detection for simultaneous determination of
Sulfachloropyridazine and Trimethoprim is developed and applied commercial veterinary
formulation for chromatographic separation, Thermoscientific Hypersil-C18, reverse phase
column was used. Separation was done using acetonitrile: pH 3.0 buffer solution (30:70, v/v)
at 0.8 ml min-1
flow rate. Analysis was done 272.0 nm at ultraviyolet detector. Under optimum
chromatographic conditions, retention time of Sulfachloropyridazine and Trimethoprim were
determined as 4.37 and 2.60 minute. The proposed method was linear in the range 1.0-100.0
μg ml-1
for Sulfachloropyridazine and 0.5-40.0 μg ml-1
for Trimethoprim. The Limit of
detection of Sulfachloropyridazine and Trimethoprim are 0.05 μg ml-1
, 0.18 μg ml-1
,
respectively. The method which is rapid, simple and does not require any separation step, has
been successfully applied to assay of commercial dosage forms containing
Sulfachloropyridazine and Trimethoprim