© 2021 Elsevier Inc.Background: Dry eye disease is one of the most common pathologies of the ocular surface. In this study, we aimed to evaluate the impact of eyelid hygiene on tear film stability, ocular symptoms, and vision-related quality of life among operating room staff. Methods: This single-blind, randomized-controlled study included a total of 142 eyes of 71 operating room staff with at least Stage I dry eye disease. The participants were divided into two groups: the intervention group (n=34) and control group (n=37). Tear break-up time, Schirmer 1 test, corneal staining grading, Ocular Surface Diseases Index, McMonnies Questionnaire, Visual Function Questionnaire were used for data collection. The intervention group received eyelid hygiene consisting of warm compress, massage, cleaning. Results: The mean age was 35.73 ± ±5.38 years in the intervention group and 38.72 ± ±6.49 years in the control group. Of the participants, 79% and 78% were females in the intervention and control groups, respectively. In the intervention group, the ocular symptoms regressed, tear break-up time, and Ocular Surface Diseases Index scores increased, the corneal staining grading decreased, ocular pain scores decreased, and mental health scores increased in the Visual Function Questionnaire subscales (p<0.05). In the control group, the tear break-up time and Ocular Surface Diseases Index scores decreased, ocular pain and existing symptoms increased, and mental health decreased (p<0.05). Conclusion: The results suggest that eyelid hygiene improves the ocular surface and tear film quality with reduced ocular symptoms and better vision-related quality of life in this population.