Akademik Veri Yönetim Sistemi
Journal of Surgery and Medicine, cilt.11, sa.9, ss.225-229, 2025 (Hakemli Dergi)
Abstract Background/Aim: Urinary tract infections (UTIs) are a significant global health concern that necessitates expedited diagnostic methods to guide appropriate antimicrobial treatment and mitigate the spread of multidrug-resistant organisms. This investigation was conducted to assess the effectiveness of two distinct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) platforms for the direct identification of UTI-causing agents from urine samples: VITEK MS (bioMérieux, France) and MALDI Biotyper Sirius (Bruker Daltonics, Germany). The findings were evaluated by comparison of the outcomes with those of conventional urine culture, which is considered the diagnostic gold standard. Methods: The study included 60 urine specimens consisting of 40 specimens from patients presenting with UTI symptoms and 20 specimens from a control group that showed no bacterial growth in culture. A differential centrifugation method was employed to prepare the samples, which were subsequently analyzed using both MALDI-TOF MS systems. Concurrently, all samples underwent conventional urine culture. Results: Among the 40 culture-positive samples, the MALDI Biotyper Sirius system demonstrated an overall identification sensitivity of 69.2% and a specificity of 95.2%. The VITEK MS system showed a sensitivity of 79.5% and a specificity of 95.2%. For the 29 samples with a bacterial concentration of ≥1×105 colony-forming units per milliliter (CFU/mL), the sensitivity was 75.9% for the MALDI Biotyper Sirius system and 79.3% for the VITEK MS system. A statistical evaluation using McNemar's test determined that the difference in sensitivity between the two platforms was not statistically significant (P=0.125). Conclusion: The findings suggest that both MALDI-TOF MS platforms have considerable promise for the rapid and direct identification of uropathogens, particularly in monomicrobial urine samples with a high bacterial load (≥1×105 CFU/mL). While the VITEK MS system exhibited a marginally higher overall identification sensitivity, the data indicate that both systems can be considered valuable assets in clinical diagnostic laboratories. Keywords: urinary tract infection, MALDI-TOF MS, VITEK MS, MALDI Biotyper Sirius, uropathogen, rapid diagnosis